Fig. 1

Expression of MEP1A in human colorectal cancer tissues and cell-lines. Relative expression of MEP1A mRNA in 36 paired tumorous samples compared with normal samples. Fold change was calculated by 2^-ΔΔCT method (a). The mean MEP1A mRNA expression in these 36 tumor tissues was significantly higher than paired adjacent normal mucosa specimens (b). Western blot analysis of MEP1A expression in seven paired colorectal tumor tissues (c). MEP1A protein is higher expressed in tumor tissues than in paired adjacent normal mucosa (d). MEP1A protein expression in five colorectal cell-lines. Grayscale values were evaluated (n = 3, * p < 0.05, compared with NCM460 cell-line) (e)