Fig. 1
From: Context dependent regulatory patterns of the androgen receptor and androgen receptor target genes
Expression data of EP156T and PrEC cells. a Agilent microarray gene expression data for the indicated gene symbols are shown in the heatmap according to supervised hierarchical cluster analysis (J-Express™ software) of different cell types with or without androgen R1881 in the growth medium. EP156T and LNCaP cells were treated with 10 nM for 48 hours and 22Rv1 and VCaP cells with 1 nM R1881 for 24 hours. Red color indicates high expression. b and c RT-qPCR comparing expression of AR, NKX3-1, TMPRSS2 and KLK3 between EP156T and PrECs. d Western Blot of AR and PSA in PrEC and EP156T cells compared to LNCaP cells with ± 1 nM R1881 stimulation for 48 hours. e RT-qPCR of AR and TP63 in EP156T cells after 6 days culture under different calcium and FCS concentrations. N.D. = not detected. Error bars show 95 % confidence intervals. RQ = relative quantity