Fig. 1

Epigenetic regulation of HTRA1 expression in HCT116 cells. a Methylation of the human HTRA1 promoter (−388 bp to −115 bp) was determined by sequencing 4 independent clones of bisulfite treated DNA of HCT116 and SW480 cells. b Mean normalized expression (MNE) of HTRA1 in SW480 and HCT116 cells determined by qRT-PCR (n = 3 independent cell lines, each done in triplicates, two-tailed Mann–Whitney U test, p-value <0.0001). c HTRA1 mRNA levels in HCT116 and SW480 cells following treatment with 5-Aza-dC and TSA determined by qRT-PCR. Results of one representative experiment are shown. MNE of control was set to 1; standard deviation was calculated from triplicates