Fig. 4
Quantification of (a, b) Ki67 immunofluorescence or (d, e) cleaved caspase 3 immunofluorescence in (a, d) RJ348 cells or (B,E) RM11A cells after treatment with a GC control siRNA or OPN-siRNA2. The percentage of Ki67 positive or cleaved caspase 3 positive cells following OPN-siRNA2 treatment are presented relative to cells treated with the GC control siRNA. c phospho-histone H3 immunofluorescence in RJ348 cells following administration of 5 μg of an OPN neutralizing antibody or 5 μg of a control antibody. The percentage of phospho-histone H3 positive cells following OPN neutralizing antibody treatment is presented relative to the control antibody. A paired t-test was used to determine statistical significance, *p˂0.05, n ≥ 3