Fig. 7

Quantification of pro-migratory and pro-inflammatory factors after chloroquine treatment. a Zymogram of MMP-2 and −9 levels after chloroquine treatment performed on protein lysates of both irradiated and non-irradiated mammary glands collected 6 h after irradiation. b Zymogram of MMP-2 and −9 levels after chloroquine treatment performed on protein lysates of D2A1 tumors implanted in pre- irradiated and non-irradiated mammary glands collected on sacrifice day (day 21). c Effect of chloroquine 40 mg/kg on the relative expression of pro-inflammatory genes potentially in mammary quantified by qPCR 6 h after the last session of irradiation. Relative mRNA expressions are plotted as a radiation enhancement ratio. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. d Summary of the proposed mechanism of chloroquine in the prevention of TNBC invasion stimulated by radiotherapy. Error bars indicate SEM. Experiments were realized in triplicate