Fig. 1

Proteomic expression of six SFs and CEA. a Representative semiquantitative immunoblot analysis of gastric and colorectal (CR) cancer (C) and their adjacent normal mucosa (NM) tissue samples. β-actin (ACTB) was used as a loading control. SW1116 cell lysate was loaded as a semiquantitative calibrator. For each SF and CEA, distinct bands in the following target molecular weights were analyzed: 29–39 kDa (HNRNPA1), 27–33 kDa (SRSF1), 20 kDa (SRSF3), 36–40 kDa (SRSF6), 33–15 kDa (SRSF7), and 180–220 kDa (CEA). b Relative quantitation of SF proteins and CEA in immunoblot analysis for gastric normal mucosa (GNM), gastric cancer (GC), colorectal NM (CRNM), CR cancer (CRC) and CR-adenoma. Each bar indicates the median and the green horizontal lines indicate the interquartile ranges. The color (compared group) matched small blocks above each median bar indicate the p value < 0.05 (by Kruskal-Wallis test followed by Conover’s post-hoc tests). c Representative subcellular distribution analysis using biochemical fractionation and immunoblot method. Whole cell (wc); nuclear extract (nu); cytosol/microsome (cyt); and membrane/organelle (me/og) fractions were loaded. The nuclear extract fraction was identified with poly (ADP-ribose) polymerase (PARP) and histone H3 (H3) and membrane/organelle fraction was identified with prohibitin. Respective subcellular fractions were normalized using the total protein quantity/ACTB. The actual experiment involved 10 paired samples (n = 20; 5 GC/GNM pairs and 5 CRC/CRNM pairs). d Representative immunohistochemical staining analysis. The actual experiment involved 10 paired samples (n = 20; 5 GC/GNM pairs and 5 CRC/CRNM pairs)