Fig. 2

MiR-410 plays a pivotal role in cholangiocarcinoma growth and invasiveness. a Overexpression of miR-410, suppresses effectively the cell growth of TFK-1 cell line. b Number of colonies (>50 μm) of TFK-1 cells treated with miR-NC or miR-410 for 48 h. c Invasion assay of TFK-1 cells treated with miR-NC or miR-410 for 24 h. d Apoptosis was evaluated by caspase 3/7, 48 h after transfection. TFK-1 cells were transfected with miR-NC, miR-410 or as-miR-410. e Evaluation of caspase-3 and PARP pathways after miR-410 overexpression (48 h post transfection) by western blot analysis. Representative pictures of Western blot analysis for cleaved caspase-3 and PARP after miR-NC and miR-410 enforced expression in TFK-1 cells. Total caspase-3 and total PARP were used as the loading control. f Tumor growth of TFK-1 cells in nude mice after i.p. treatment with miR-NC or miR-410. Arrows indicate the administration of miRNAs (days 15, 20, 25 & 30)