Fig. 6From: 3D culture of Her2+ breast cancer cells promotes AKT to MAPK switching and a loss of therapeutic responsegrowth in 3D promotes loss of PI3K/AKT and gain in MAPK signaling. Cells were growth in 2D or 3D culture, lysed and proteins analyzed by Western blotting. 3D culture resulted in a significant loss of PI3K/AKT pathway activity and a gain in MAPK (BT474) and MEK (BT474 and MDAMB361 cells)Back to article page