Fig. 3

L-Arg affected two different populations of macrophages in the spleens of 4 T1 TB mice. Two populations of macrophages (CD11b⁺F4/80⁺ and CD11b⁻F4/80⁺ gated in Gr-1) were shown (a). Total RNA was purified from tumor block and ARG-1 and iNOS mRNA were quantified by real-time RT-PCR (b). NO levels from the supernatant of cultured splenic cells were evaluated by the Griess reaction (c). * P < 0.05 and ** P < 0.01 compared to PBS group. ^## P < 0.01 compared to L-Arg + AG group