Fig. 4

Potentiation of ephrin-A1-induced cell rounding and retraction fiber formation by FVIIa is independent of PAR2. MDA-MB-231 cells were pretreated with 50 μg/ml anti-TF antibody 10H10 or 100 μg/ml PAR2-blocking antibody for 30 min, stimulated with 10 nM FVIIa for 1 h and then 1 μg/ml ephrin-A1 for 10 min. Alternatively, 10 nM active-site inhibited FVII (FFR-FVII) was added instead of FVIIa. Potentiation of cell rounding and retraction fiber formation was not prevented by the anti TF 10H10 antibody (43.4 ± 4.2 % vs 16.6 ± 9.2 %, p = 0.01) or a PAR2-blocking antibody (31.4 ± 9.9 % vs 8.1 ± 4.6 %, p = 0.02), FFR-FVII did not increase cell rounding and retraction fiber formation together with ephrin-A1 (14.3 ± 5.7 % vs 14.4 ± 1.2 %, p = 0.99). N = 3, results are expressed as means ± SD of the percentage of rounded cells with retraction fibers. Representative images are shown