Fig. 2

Overexpression of TF is required for EphA2 co-localization and cleavage in U251 cells. a-b U251 cells were transfected with a plasmid encoding human TF or treated with Lipofectamine alone as control. The figure shows confocal micrographs of control (a) or TF transfected (b) U251 cells stained for EphA2 (green signal) and TF (red signal). Blue signal represents DAPI-stained DNA. Scale bar represents 20 μm. c Lipofectamine-treated or TF-transfected U251 cells were treated with 10 nM FVIIa for the indicated time points. Samples were analyzed for EphA2 and TF expression by Western blot. N = 2–3, representative blots are shown