Fig. 2

BRCA1 determines the frequency of stem cells or EMT marker expressing cells within cell lines. a The distinct CD44^high/24^–population (represented by the black arrow) possessing mesenchymal properties is observed within the CD44⁺/24^–/low cells (P4) in HCC1937/wt BRCA1. b Such a population is not discernable within the HCC1937 cell line. c Marker profiling of mammospheres shows better expression of EMT markers (Snail, Slug, Vimentin) and mesenchymal marker (α-SMA) by HCC1937/wt BRCA1 cells. Expression of stem cell markers (Nuclear Oct 4 and membrane bound β-catenin) was more prominent in the HCC1937 cell line as observed by immunofluorescence as well as western blotting. Scale = 50 μm. “Ah” and “Mm” in western blot represents Adherent and Mammospheres respectively