Characterisation of the DNA sequence specificity, cellular toxicity and cross-linking properties of novel bispyridine-based dinuclear platinum complexes

Table 1 Summary of the interstrand cross-linking efficiency of the dinuclear platinum compounds experimental values and is reported along with its corresponding SEM value

Compound	ICLE (%)	Drug concentration at 50 % dsDNA retention (μM)	r_b (drug : nucleotide) at 50 % dsDNA retention	Last detected data point (μM)
Cisplatin	6.8 ± 0.5	0.09 ± 0.01	1.9E-3 ± 1.4E-4	10.0
1,8 platinum	43.5 ± 19.0	0.02 ± 0.01	4.7E-4 ± 3.1E-4	0.3
1,10 platinum	23.1 ± 3.9	0.03 ± 0.01	5.7E-4 ± 9.6E-5	0.3
1,12 platinum	14.2 ± 6.4	0.06 ± 0.02	1.1E-3 ± 5.0E-4	1.0

The compounds have been arranged vertically in the table alongside their corresponding ICLE values, expressed as a percentage. The drug concentration (expressed in μM) that induced a 50 % retention of dsDNA, is reported alongside its respective r_b value. The amount of nucleotide sample incubated with each compound was 2 x 10^-9 moles, whereby this value was used to calculate the r_b value. The ‘last detected data point’ refers to the highest drug concentration whereby the DNA was still detectable on the agarose gel. All values are reported in the table with their respective SEM values, which were determined from three separate experiments

ISSN: 1471-2407