Fig. 1

HAS1 and HAS2 immunoreactivity in superficially and deeply invasive melanomas and in lymph node metastases. Immunostainings of HAS1 a-c and HAS2 d-f in superficially a and d and deeply invasive melanomas b and e and in lymph node metastases c and f. Black dash lines in a and d mark the border between the tumor and the stroma. Black asterisk in a points to numerous tumor infiltrating lymphocytes in superficial melanoma. Black arrows a, d indicate melanin containing tumor cells in superficial melanoma and black arrowheads in (D, insert) point to HAS2 immunopositive stromal cells. In deep melanoma and lymph node metastasis tumor cells show weak immunostaining or are totally negative b, c, e, f. Scale bars 100 μm