Fig. 4

Effect of esiRNA HMOX1/2 transfection on HMOX expression in pancreatic cancer cells. PA-TU-8902, MiaPaCa-2 and BxPC-3 cells were transfected with esiRNA for HMOX1/HMOX2 or universal control esiRNA and mRNA expression of a) HMOX1 and b) HMOX2 was measured after 24 h. c HMOX1 protein expression was measured in cells treated with 30 μM hemin for 20 h. Hemin treatment was used to upregulate HMOX1 protein expression to cumulate detectable levels of HMOX1 protein. *p < 0.05, **p < 0.01, ****p < 0.0001 vs. esiRNA control cells