Fig. 8

Treatment of MM cells, regardless of their IMiD-sensitivities, with AGO2-shRNA induced apoptosis. a Profiles for apoptosis analysis with Alexa Fluor 647 Annexin V. MM cells were treated with either DMSO (as a control) or 10 μM lenalidomide (LEN) for six days; or with either lentiviral vector (as a control) or AGO2-shRNA74 (sh74) for four days, respectively. The cells were stained with annexin V-Alexa Flour 647 and 0.5 mg/mL propidium iodide (PI) and then analyzed by flow cytometry. Region 1 indicates the live cells; 2, necrotic cells; 3, late apoptotic cells; 4, early apoptotic cells. b Effects of lenalidomide on MM cells. Annexin V⁺ indicates annexin V positive cells. PI⁺ indicates propidium iodide positive cells. The bar graph indicates the mean ± standard deviation. *P value < 0.05; **P value < 0.01. c Effects of AGO2-shRNA74 on MM cells