Fig. 7

Matrigel™ environment amplifies dependence upon AIF-mediated growth and metabolism. Matrigel™ growth: Cells were plated in replicate Matrigel™ layers and allowed to grow for up to 21 days. Representative images (captured at 10× magnification) are shown (Panel a). Cells were extracted from substrate and quantified by Coulter™ counting (Panel b). Glucose consumption in Matrigel™ substrate (Panel c): Cells were exposed to Matrigel™ conditions prior to media collection and total glucose measurements using the QuantiChrom™ Glucose Assay Kit (BioAssay Systems). Cells were extracted from substrate with the Matrigel Recovery Solution (Corning) and quantified by Coulter™ counting. Total numbers of cells in each well were used to determine glucose consumption per cell. Data are shown as average ± standard deviation. *p < 0.05