Fig. 2
From: Basal metabolic state governs AIF-dependent growth support in pancreatic cancer cells
Establishment of AIF-deficient pancreatic cancer cell lines. PANC-1 and MIA PaCa-2 cells were stably infected with shRNA hairpins targeting LacZ or AIF with GFP as a selectable marker via lentiviral delivery. GFP positivity of infected cell lines was assessed by flow cytometry (Panel a) and fluorescence microscopy (Panel b). Equivalent targeting in BxPC-3, HPAC, and HPAF-II cells was achieved by puromycin selection; suppression of AIF protein expression was verified by immunoblot analysis (Panel c)