Fig. 3

Effects of KC-53 on cell apoptosis and DNA integrity. a Cells were treated with 0 or 5 μΜ KC-53 for the indicated time points and apoptosis was assessed with Annexin-V/PI staining. Statistical significance was determined by comparing treated samples with the corresponding population of the vehicle control. For comparison, Doxorubicin (Dox) at 0.5 μΜ was used as positive control. b (i) Cells were treated with 0 or 5 μΜ KC-53 in the presence or absence of 20 μM z.vad.fmk for 24 h. The presence of nucleosomes in the cytoplasm was determined with the ELISA cell death detection kit and is expressed as Enrichment Factor. Etoposide (Eto) at 5 μΜ was used as positive control. (ii) Cells were treated with vehicle control or 5 μΜ KC-53 in the presence or absence of 20 μM z.vad.fmk, as shown, for 24 h. Cell viability was assessed with the MTT assay. The results represent the mean ± SEM of two replicates and are representative of three independent experiments. (*p value <0.05, **p value <0.01, ***p value <0.001, #p value <0.05, ###p value <0.001)