Fig. 4

Effects of melflufen in subcutaneous DOHH-2 xenografted mice. Presented as tumor volume (a), survival (b) and body weight change (c). Mice in control group (n = 5) gained weight and showed no signs of toxicity, but individuals were prematurely sacrificed due to tumor size on day 33 (n = 2) and 35 (n = 2). Animals in vincristine control group had excellent tumor control but lost weight and one animal was sacrificed on day 25, one on day 33 due to >20 % weight reduction. Animals treated with melflufen had no weight loss but significant tumor growth reduction on day 33 (p < 0.05). Log-rank test showed a significant difference in survival between the control group and the melflufen treated group (p = 0.0144). In A and C mean values with SEM are displayed