Fig. 7
From: Epigenetic silencing of miR-181c by DNA methylation in glioblastoma cell lines
Depletion of CTCF in U87MG cells affects the transcription levels of NOTCH2. a NOTCH2 mRNA levels in U87MG cells transduced with an inducible shRNAi against CTCF after 3 and 30 days with or without DOX induction (3d shRNAi-DOX; 3d shRNAi + DOX; 30d shRNAi-DOX; 30d shRNAi + DOX). Empty vector controls are shown (3d EV-DOX and 3d EV + DOX). *p-value < 0.05. N.S., not significant. This set of data is representative of three-independent experiments. b In U87MG cells CTCF binds to the promoter region of miR-181c (Left). Loss of CTCF causes epigenetic silencing of miR-181c by DNA methylation and an increase of NOTCH2 mRNA (Right)