Fig. 3
From: Epigenetic silencing of miR-181c by DNA methylation in glioblastoma cell lines
In vivo CTCF binding to the promoter region of the miR-181c. Chromatin immunoprecipitation against CTCF in U87MG, T98G glioblastoma cells and K562 erythroleukemic cells are shown. The enrichment was evaluated in what we designated as the CTCF-upstream and the CTCF-downstream predicted sites in relation to the transcription start site of primiR-181c. The black arrows of the scheme show the location of primers used for PCR amplification. The linear range of Input DNA amplification products is shown. Igf2/H19 DMR was used as a positive control for CTCF in vivo enrichment. This set of data is representative of at least three independent experiments