Skip to main content
Fig. 5 | BMC Cancer

Fig. 5

From: Apoptosis-induced ectodomain shedding of hypoxia-regulated carbonic anhydrase IX from tumor cells: a double-edged response to chemotherapy

Fig. 5

Doxorubicin-induced apoptosis is associated with reduced CA IX expression and increased CA IX ECD shedding in normoxic cells. a FACS analysis of the subG1 fraction in the control and DOX-treated cells determined by the DNA labeling with propidium idodide, b and corresponding proportions of the M75-labeled CA IX-positive cells in the same cell samples. c Percentage of the CA IX- and CA IX+ cells within the DOX-treated dead cells. Data show that the dying cells are principally CA IX-negative. d Increased levels of the CA IX ECD shed from the DOX-treated versus control normoxic cells. Grey lines illustrate the DOX-related fold induction of the CA IX ECD shedding. Error bars represent the mean ± SD. **p < 0.01, *p < 0.05

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us