Fig. 4

The expression levels of CAPZB and cell growth in INI1-overexpressing EpiS cells. a In the VAESBJ cell line, the INI1 expression was induced in the doxycycline (Dox)-induced INI1-overexpressing cells, whereas the other three cells did not show an expression of INI1 (a, left panel). In the ESX cell line, all four cells expressed INI1, and the Dox-induced INI1-overexpressing cells had higher expression levels than the other three cells (a, right panel). Regarding the expression levels of CAPZB, the WB assay demonstrated no remarkable differences among the four types of cells for both the VAESBJ and ESX cells. b In the cell proliferation assay, a significant growth inhibition was observed in the Dox-induced INI1-overexpressing cells (p < 0.01, B, left panel). The cell proliferation assays of the ESX cells showed no significant differences in growth between the INI1-overexpressing cells and the control cells (b, right panel). c Relative expression of CAPZB mRNA in the VAESBJ and ESX cells (right and left panel, respectively). The mean expression levels of CAPZB in the RFP DOX-, RFP DOX+, INI1 Dox- and INI1 Dox + cells of each cell line, as determined using real-time PCR are shown, normalized to the expression of CAPZB mRNA in the RFP DOX- cells. In the VAESBJ cells, the INI1 Dox + cells had higher mRNA expression levels of CAPZB than the other three cells (c, left panel). On the other hand, in the ESX cells, there were no significant differences in the mRNA expression levels of CAPZB among the four cells (c, right panel)