Fig. 7
From: Relevance of miR-21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells
Apoptosis analysis of cervical cancer cells by silencing of miR-21. SiHa cells were non-transfected or transfected with pSilence1.0-U6 or pSIMIR21 plasmids and were harvested and fixed in 70Â % ethanol. Cells were treated with propidium iodide and approximately 100,000 nuclei were processed by FASC-Sort. The assays were normalized with non-transfected SiHa cells and the data were analyzed with ModFitLT software