Fig. 6
From: Relevance of miR-21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells
Epifluorescence staining with Acridine Orange (AO) and Propidium Iodide (PI) in response to miR-21 silencing. SiHa (panel a) and HeLa cells (panel b) were plated at 70 % confluent on glass coverslips. Attached samples of SiHa and HeLa cells on a slide were harvested at 48 h after transfection with pSilencer 1.0-U6 or pSIMIR21 plasmids and stained with 5 μg/ml of AO and PI dyes. Apoptosis control was induced by 5 μM of H2O2 added to cell culture during 2 h. Following transfection, cells were observed in a Nikon Elipse 400 epifluorescence microscopy and samples were analyzed by FITC/TRITC using the 40X Fluor objective