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Table 2 The primers of 3 BDNF mRNA variants and β-actin used in this study

From: More expression of BDNF associates with lung squamous cell carcinoma and is critical to the proliferation and invasion of lung cancer cells

mRNA   Sequences Annealing temperature
hBDNF_IVS forward 5′-GCTGCAGAACAGAAGGAGTACA-3′ 61.8 °C
reverse 5′-GTCCTCATCCAACAGCTCTTCTATC-3′
hBDNF_VIS forward 5′-GGCTTTAATGAGACACCCACCGC-3′ 63.3 °C
reverse 5′-GTCCTCATCCAACAGCTCTTCTATC-3′
hBDNF_IXS forward 5′-TTTCTCGTGACAGCATGAGCAG-3′ 60.6 °C
reverse 5′-GTCCTCATCCAACAGCTCTTCTATC-3′
β-actin forward 5′-TGAGTCTCCTTTGGAACTCTGC-3′ 55 °C
reverse 5′-TAGCAACGTACATGGCTGGG-3′