Fig. 6

FN assembly is impaired in shFN1 cells cultured in 3D lrECM a Representative images of aggregates 2 days after exogenous FN conjugated to Rhodamine (Rho) is added to 3D lrECM culture (inset shows no FN assembly on day 0). IgG control and integrin-blocking peptides were compared in shFN and Scr cells. Scale bar represents 20 μm. b Immunoblots show that FN and phospho-p38, but not other proliferation markers in the presence of blocking peptides. c Graph show a reduction in the number of shFN2 colonies that assemble exogenous FN as a cortical ring compared to Scr colonies d Drug uptake is only modestly slower as determined from fluorescent recovery after photobleaching. Graph showing that the recovery time for drug influx is only modestly slower for shFN2 3D aggregates. Conditioned media to probe FN and TNC was run on separate gels where equally loaded protein