Fig. 1
From: Binimetinib inhibits MEK and is effective against neuroblastoma tumor cells with low NF1 expression
Neuroblastoma patient samples and cell lines show expression and activity of components of the RAS/MAPK signaling pathway. a Neuroblastoma patient samples were lysed and Western blots for total MEK, phosphorylated MEK (p-MEK), total ERK, and phosphorylated ERK (p-ERK) were performed. Vinculin was used as a loading control. b A panel of nine neuroblastoma cell lines, HeLa cells and 293T cells were lysed and Western blots for total MEK, p-MEK, total ERK, and p-ERK were performed. Actin and vinculin were used as loading controls. c, d Relative MEK, p-MEK, ERK, and p-ERK western blot band intensities were determined and plotted for each tested tumor sample and cell line