Fig. 6

Early and late markers of apoptosis in A2780 cells treated with 0.2 μM docetaxel.A2780 cells were treated with 0.2 μM docetaxel (DXL) for 8, 24, 48 and 72 h. a Cells were stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. Scatter plots of stained cells at each time point are shown. b A2780 cells were stained with propidium iodide only and analyzed by flow cytometry. Histograms of stained cells are shown for each time point. c DNA laddering in A2780 cells treated with 0 and 0.2 μM docetaxel for times ranging from 2 to 72 h. Jurkat cells were treated with 5 and 30 μM etoposide as a positive control. DNA was isolated from cells at each time point and analyzed by agarose gel electrophoresis. A representative ethidium bromide stained gel is shown