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Fig. 5 | BMC Cancer

Fig. 5

From: The anti-oxidative transcription factor Nuclear factor E2 related factor-2 (Nrf2) counteracts TGF-β1 mediated growth inhibition of pancreatic ductal epithelial cells -Nrf2 as determinant of pro-tumorigenic functions of TGF-β1

Fig. 5

Nrf2 hardly impact on basal and TGF-β1 induced apoptosis in pancreatic ductal epithelial cells. a, d, e H6c7-pBp, H6c7-kras and Colo357 cells were transfected either with control siRNA or Nrf2 siRNA or (b, c) H6c7-pBp and H6c7-kras cells were transfected with a control vector (pcDNA3.1) or Nrf2-HA. Then, cells were either left untreated or were treated with 10 ng/ml TGF-β1 for 48 h. Caspase-3/7 activity was determined and normalized to the cell number. a Basal caspase-3/7 activity of control siRNA, untreated H6c7-pBp, H6c7-kras and Colo357 cells, expressed as n-fold caspase-3/7 activity of H6c7-pBp cells which were set as 1. b Caspase-3/7 activity in H6c7-pBp and H6c7-kras after overexpression of Nrf2-HA or (d) siRNA mediated Nrf2 suppression, expressed as n-fold caspase-3/7 activity of the corresponding control transfected cell line which was set as 1. c + e Caspase-3/7 activity in TGF-β1 treated H6c7-pBp and H6c7-kras after (c) overexpression of Nrf2-HA or (e) siRNA mediated Nrf2 suppression, expressed as n-fold caspase-3/7 activity of the corresponding control transfected, TGF-β1 treated cell line which was set as 1. Data are presented as mean ± SD of 3-5 independent experiments. * = p < 0.05

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