Fig. 5
From: IκBα mediates prostate cancer cell death induced by combinatorial targeting of the androgen receptor
The effect of combinatorial AR targeting on NFKBIA levels and p53 signaling. a The expression of NFKBIA over a timecourse was normalized to GUSB and HPRT1. Relative expression is the fold change expressed relative to vehicle control. Values indicated are the mean of technical and biological replicates ± SEM, and are representative of three independent experiments. * = p < 0.05 using one-way ANOVA with Bonferroni post-hoc test, compared with bicalutamide or vorinostat individually. b Western blot analysis of lysates from LNCaP cells cultured with either vehicle control [VEH], 1 μM vorinostat [VOR], 2.5 μM bicalutamide [BIC] or the combination of 1 μM vorinostat and 2.5 μM bicalutamide [BIC+VOR] for 12, 48, or 96 h. Steady state levels of IκBα are shown, and tubulin is shown as a loading control. c mRNA expression levels of TP53INP1 and CDKN1A are shown as for NFKBIA. * = p < 0.05 using one-way ANOVA with Bonferroni post-hoc test