Fig. 1
From: METCAM/MUC18 is a novel tumor and metastasis suppressor for the human ovarian cancer SKOV3 cells
Expression of METCAM in various human ovarian cancer cell lines (a) and in G418R- clones derived from SK-OV-3 (b). a The expression of METCAM/MUC18 in the lysates from various cells lines was determined by Western blot (WB) analysis as described in “Methods”. Cell lysate from a human melanoma cell line, SK-Mel-28, was used as a positive control (lane 1) and those from human ovarian cancer cell lines, BG-1 (lane 3) and SK-OV-3 (lane 6) as negative controls. METCAM/MUC18 expression levels in cell lysates from one immortalized human ovarian epithelial cells (IOSE) and in five human ovarian cancer cell lines are shown in lanes 2 to 7. The number under each lane indicates the relative level of METCAM/MUC18 of each cell line, assuming that in SK-Mel-28 is 100 %. Only the house-keeping genes, actin and GAPDH, are shown here as the loading controls. b Human METCAM/MUC18 expression in lysates prepared from various clones/cells was determined by Western blot analysis as described in “Methods”. METCAM/MUC18 expression level in cell lysates from a human melanoma cell line, SK-Mel-28, was used as a positive control (lane 1) and from the parental human ovarian cancer cell line, SK-OV-3, as a negative control (lane 2). METCAM/MUC18 expression in cell lysates from one single SK-OV-3 clone (METCAM Clone 2D-9) and two pooled SK-OV-3 clones (METCAM Clone 2D and Control (Vector) Clone 3D) are shown in lanes 3–5. Both the METCAM Clone 2D-9 and the METCAM Clone 2D were derived from SK-OV-3 cells transfected with the human METCAM/MUC18 cDNA gene. The Control (Vector) Clone 3D was from SK-OV-3 cells transfected with the empty vector. The number under each lane indicates the relative level of METCAM/MUC18 of each cell line, assuming that in SK-Mel-28 was 100 %. β-tubulin is shown as the loading control