Table 1 Sequence of primers used for quantitative real-time PCR
Gene | 5′-3′ Sequence |
|---|---|
TNF-α | Forward: CCCTCACACTCAGATCATCTTCT |
Reverse: GCTACGACGTGGGCTACAG | |
IL-1α | Forward: CGAAGACTACAGTTCTGCCATT |
Reverse: GACGTTTCAGAGGTTCTCAGAG | |
IL-6 | Forward: TAGTCCTTCCTACCCCAATTTCC |
Reverse: TTGGTCCTTAGCCACTCCTTC | |
CXCL9/MIG | Forward: GGAGTTCGAGGAACCCTAGTG |
Reverse: GGGATTTGTAGTGGATCGTGC | |
CXCL10/IP-10 | Forward: CCAAGTGCTGCCGTCATTTTC |
Reverse: GGCTCGCAGGGATGATTTCAA | |
CCL5/RANTES | Forward: GCTGCTTTGCCTACCTCTCC |
Reverse: TCGAGTGACAAACACGACTGC | |
IL-1β | Forward: GCAACTGTTCCTGAACTCAACT |
Reverse: ATCTTTTGGGGTCCGTCAACT | |
Ocln | Forward: TTGAAAGTCCACCTCCTTACAGA |
Reverse: CCGGATAAAAAGAGTACGCTGG | |
ZO-1 (TJP1) | Forward: GCCGCTAAGAGCACAGCAA |
Reverse: TCCCCACTCTGAAAATGAGGA | |
Cldn3 | Forward: ACCAACTGCGTACAAGACGAG |
Reverse: CAGAGCCGCCAACAGGAAA | |
GAPDH | Forward: CTGCACCACCAACTGCTTAG |
Reverse: GGGCCATCCACAGTCTTCT |