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Fig. 1 | BMC Cancer

Fig. 1

From: KSHV gB associated RGD interactions promote attachment of cells by inhibiting the potential migratory signals induced by the disintegrin-like domain

Fig. 1

Generating KSHV gBΔD. a The diagram shows the schematic of orf8 encoding the full length gB (gB/pCDNA3.1) compared to gB lacking a functional DLD (gBΔD/pCDNA3.1) and RGD (gBΔR/pCDNA3.1), respectively. b In vitro transcription and translation of KSHV orf8. IVT was performed using pCDNA3.1 (lane 1, 6), gB/pCDNA3.1 (lane 2, 7), gBΔD/pCDNA3.1 (lane 3, 8), gBΔR/pCDNA3.1 (lane 4, 9), and gH/pCDNA3.1 (lane 5, 10) using the microsomal membranes. The IVT products were immunoprecipitated using either rabbit anti-gB antibodies (lanes 1 – 5) or pre-immune IgGs (lanes 6 – 10). The immunoprecipitates were resolved on a 10 % SDS-PAGE gels and autoradiographed. The letters ‘G’ and ‘NG’ denote glycosylated and non-glysoylated forms of gB

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BMC Cancer

ISSN: 1471-2407

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