Fig. 5

Xenografts show signs of metastasis. a. Macroscopically subarachnoidal tumor dissemination is seen frequently at tumor progression and b. tumor cells can then constantly be found in the CSF (HE stained gross section). c. Xenograft cytospin of subarachnoidal disseminated tumor cells at the humane endpoint reveal blue-cell tumor cells with for LCA histology typical cell wrapping (arrow). d. Histology (HE-staining) indicates aggressive, infiltrative behavior with xenografted tumor cells invading from the brain surface (arrow head) and the Virchow-Robin spaces (arrows). e. Vimentin-staining showing tumor cells with pseudopodia reaching from the brain surface into the brain tissue