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Fig. 3 | BMC Cancer

Fig. 3

From: Breast cancer stem cell selectivity of synthetic nanomolar-active salinomycin analogs

Fig. 3

Treatment with the analogs 2ac induce expression of E-cadherin and β-catenin at the cell surface. The cells were treated with a 50 nM concentration of the compounds for 72 h. ac E-cadherin, β-catenin and vimentin, respectively, detected with immunofluorescence microscopy after appropriate labeling with specific primary antibodies followed by secondary Alexa Fluor 488-conjugated antibodies (green). Nuclei were stained with bisbenzimide (blue). Bars = 20 μm. SA: salinomycin, 2a: carbamate, 2b: acetate, 2c: carbonate and 3: C1-methyl ester

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