Fig. 2
From: Select Bcl-2 antagonism restores chemotherapy sensitivity in high-risk neuroblastoma
ABT-199 activates the intrinsic mitochondrial apoptosis pathway in Bcl-2 dependent NBs. a Following a 24 h exposure to increasing concentrations of ABT-199, both SMS-SAN and NB-1643 (Bcl-2 dependent) show activation of caspases 3 and 7. Mcl-1 dependent cell line, IMR5 does undergo apoptosis in response to the cytotoxic drug, etoposide, but fails to activate caspases 3 and 7 in response to ABT-199. b Western blot evidence for downstream PARP cleavage following a 48 h ABT-199 treatment of SMS-SAN. c Following 24 h treatment with etoposide or ABT-199, NB cells were analyzed for Annexin V positivity, an indicator of cell membrane externalization of phosphatidylserine and late stage apoptosis