Fig. 4

Effects of RAGE expression on xenografted tumour growth in nude mice. The xenografted tumour volume (a and b) and diameter (d and e) of nude mice transfected with control or RAGE-knockdown HEC-1A cells was shown. Magnification is 200×. c, summary of xenografted tumour volume at 0, 5, 10, 15, 20 days after transfection with HEC-1A cells (control) or RAGE-knockdown HEC-1A cells. F, xenografted tumour weight at 20 days in transfected control or RAGE-knockdown HEC-1A cells. g and h, sections were subjected to immunostaining for Ki67 in xenografted tumour with transfected control or RAGE-knockdown HEC-1A cells. Magnification is 200×. i, summary of the Ki67 levels from the measurements shown in g and h. Each group, n = 12. Bar graphs show mean ± SD. * P < 0.05 vs. control