Fig. 3

Effects of RAGE on microvessel density. a, western blot of RAGE expression before and after knockdown by siRNA in HEC-1A cells. b and c, sections subjected to immunostaining for RAGE in xenografted tumours of transfected control or RAGE-knockdown HEC-1A cells. Magnification is 100×. d and e, sections subjected to immunostaining for microvessel density in xenografted tumours of transfected control or RAGE-knockdown HEC-1A cells. Magnification is 400×. f, summary of microvessel density from the measurements shown in D and E. Each group, n = 12. Bar graphs display mean ± SD. * P < 0.05 vs. control