Fig. 3
From: Tumor slice culture system to assess drug response of primary breast cancer
Induction of cell death after prolonged culture of tumor slices is minimal. a Representative images of tumor slices from the same tumor displayed similar TUNEL staining intensities at 2 hours, 4 days and 6 days of incubation. Tumor slices incubated with high concentrations (10 μg/ml) of the chemotherapeutic compound Cisplatin revealed massive TUNEL signal. This specific signal was regarded as the positive control for TUNEL signal. Blue = DAPI, Green = TUNEL. Scale bars represent 100 μm. b Quantification of TUNEL in different tumor slices. TUNEL signal is variable due to tumor heterogeneity. Each black dot represents one image field. For each image field the percentage of TUNEL-positive DAPI pixels is given. Error bars indicate interquartile range and blue bars represent median values. c TUNEL signal was determined for multiple tumors after short and prolonged incubation. Incubation times varied per tumor depending on availability of tumor slices. Black dots indicate median values. Error bars indicate interquartile range