Fig. 5

Forced KDM5B expression induced tumorigenicity, enhanced migration, and acquisition of CSC-like phenotype in non-tumorigenic MCF-10A breast cancer cell line. a Western blot showing cytoplasmic and nuclear expression profile of KDM5B compared with that of snail and vimentin in MDA-MB-231 WT, MDA-MB-231 KD, MCF10A WT and MCF10A OE. b A representative RT-PCR result showing transcript expression of KDM5B, MALAT1, snail, vimentin, and miR-448. GAPDH served as loading or internal control. c Mammosphere formation assay showing the effect of induced KDM5B expression in MCF10A cells. MCF10A WT and MDA-MB-231 WT cells were used as negative and positive control, respectively. d, e Quantitative box-plots depicting mammosphere formation ability and efficiency in MDA-MB-231WT, MDA-MB-231shKDM5B, MCF10AWT and MCF10AoeKDM5B. f, g Representative Photomicrogram and bar chart of invasion assays showing the effect of KDM5B overexpression in MCF10A cells. ***P < 0.001