Fig. 1

Establishment of transgelin-overexpressing RKO cell lines. a. Phase contrast images of unmodified RKO cells (RKO^WT) and derivatives stably transfected with empty vector (RKO^CTRL) or with transgelin cDNA (RKO^TAGLN). Inset, confocal fluorescence imaging showing the transfection marker, EmGFP. b. Immunoblot analysis of total cell extracts from RKO^CTRL or RKO^TAGLN cells. c. Fold change in transgelin mRNA expression as determined by real-time PCR analysis, normalized to GAPDH. Mean of three experiments, error bars denote standard deviation. P value determined by Student’s t-test. d. Anti-transgelin immunostaining of RKO^CTRL or RKO^TAGLN cells. Cells were counterstained for DNA with DAPI; merged image is indicated. Scale bar, 5 μm