Fig. 3

Representative gene marker expression in HepG2 and fused cells. a Flow cytometer analysis for the expression of marker protein AFP and CD133 in HepG2, fused cells, and hESCs. Cells in the control group were not antibody incubated while cells in the treated group were incubated with AFP and CD133 antibody. As hESCs were labeled with GFP, hESCs from both the control and treated groups were green positive population. b Gene marker expression analysis shows that both AFP and CD133 were expressed in the fused cells, indicating that both HepG2 and hEScs affected the gene expression in the fused cells