Fig. 2

Promoter hypermethylation of DUSP2 in human cancers. a. Combined bisulfite restriction analysis (COBRA) of DUSP2. Bisulfite-treated DNA from the indicated cancer cell lines, normal epithelial breast cells (HB2) and in vitro methylated DNA (ivm) was amplified, digested with TaqI (+) or mock digested (-) and resolved on 2 % agarose gels with a 100 bp marker (M). Methylation levels obtained from pyrosequencing are indicated in percentage. b. Bisulfite pyrosequence analysis of DUSP2 in human cells. The mean methylation levels of five CpGs were analyzed by pyrosequencing (Seq1). The analysis included the results of three independent experiments. The dashed line marks 5 % threshold