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Fig. 1 | BMC Cancer

Fig. 1

From: Both Talin-1 and Talin-2 correlate with malignancy potential of the human hepatocellular carcinoma MHCC-97 L cell

Fig. 1

Lentivirus-mediated stable Talin-1 (TLN-1) knockdown in the minimally metastatic HCC MHCC-97 L cells. Blank: non-transduced MHCC-97 L cells; TLN-1 shRNA: MHCC-97 L cells transduced with lentivirus-mediated TLN-1 shRNA; Scramble shRNA: MHCC-97 L cells transduced with lentivirus-mediated scramble shRNA. a According to fluorescence microscopy, transduction efficiency in MHCC-97 L cells (the percentage of GFP-positive cells) was >70 % at 72 h (magnification: ×200). b TLN-1 mRNA expression levels were determined by real-time RT-PCR at 72 h after transduction, with GAPDH used as an internal control. c TLN-1 protein expression levels were determined by Western blot at 72 h after transduction. β-actin was used as an internal control. Data are mean ± Standard deviation (SD). **P < 0.01 vs. scramble shRNA

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