Fig. 5

Deferasirox induced hypoxia-inducible factor -1α (HIF-1α) protein stabilization. Levels of hypoxia-inducible factor -1α (HIF-1α) protein and heat shock cognate protein 70 (HSC70) protein used as loading control protein in U251 glioblastoma cells cultivated at 21 % of oxygen or 3 % of oxygen (a) and in U87 glioblastoma cells cultivated at 21 % of oxygen or 3 % of oxygen (b) in untreated condition (CONT) or 3 days after treatment with 10 μM of deferasirox (DFX) in non-irradiated condition (0 Gy) or following irradiation with 16 Gy (16 Gy). Western blot data represent one of three independent experiments with comparable results