Fig. 3
From: Molecular characterization of irinotecan (SN-38) resistant human breast cancer cell lines
Western blotting of total protein extracts from SN-38 resistant (R) in comparison to their respective controls (Parental (P) and DMSO (D)). All samples were immunoblotted with an antibody to β-actin to illustrate equal protein loading (a): Western blotting of Top1/β-actin; (b): BCRP/β-actin; (c): MDR1/β-actin. Protein bands were quantified by image J software. Number represents relative intensity (RI) bands to their respective DMSO control. d Top1 and β-actin proteins signals from nanocapillary electrophoresis were quantified and expressed in percent of the relevant DMSO control cell lines. All differences were signicant (p < 0.05) with p-values of 0.0088, 0.00012, 0.0017 and 0.00018 for MDAacq, MCF-7acq, MDAde novo and MCF-7de novo, respectively. e Visualization of the Top1 isoelectric patterns in the cell lines MCF-7acqDMSO, MCF-7acq (top panel) and MDAacqDMSO, MDAacq (lower panel)