Fig. 3

Phosphorylations of Akt and Erk are downregulated by c-Met inhibitors only in c-Met overexpressed cell lines. Hs746T (a), or AGS (b) was treated with c-Met inhibitors or crizotinib in a dose dependent manner for 3 h. Cell lysates were prepared for immunoblot with phospho antibodies of c-Met, Akt, and Erk. Tubulin band shows equal loading. c-Met phosphorylation was quantified by imageJ software