Fig. 8

2c-induced expression of Beclin1 and LC3B mRNA. 2c treated and untreated HT-29 cells were subjected to RNA isolation and the expression of mRNA was analysed. Beclin1 and LC3B expression in HT-29 cells treated with or without 2c were measured by qRT-PCR and normalized to GAPDH expression. Data are expressed as fold change relative to untreated cells showing induction of autophagy in cells treated with 2c for different time intervals (12, 24 and 48 h)